Overexpression of catalytic subunit M2 in patients with ovarian cancer / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>The formation and growth of tumors are related to the synthesis of the DNA. The enzyme ribonucleotide reductase (RR) is an enzyme that regulates the total rate of DNA synthesis and thus plays a pivotal role in cell growth. Catalytic subunit M2 (RRM2) is the main unit modulating the ribonucleotide reductase enzymatic activity. This study aimed to investigate the expression of RRM2 mRNA and protein in patients with ovarian cancer and its relevance to diagnosis and clinical outcome of the patients.</p><p><b>METHODS</b>RRM2 mRNA levels and protein expression were detected in 98 ovarian specimens with immunohistochemistry and real-time quantitative polymerase chain reaction (PCR). Expression of the RRM2 protein and correlation of the RRM2 gene expression with clinical pathological features were analyzed. The Kaplan-Meier test was used for evaluating RRM2 expression and time to progression and survival. The Cox proportional model was used to analyze the risk factors in prognosis of patients.</p><p><b>RESULTS</b>Positive RRM2 immunostaining was found in 43 of 62 (69.4%) patients with epithelial ovarian cancer, 10 of 15 (66.7%) patients with borderline neoplasm, 4 of 15 (26.7%) patients with benign growths, and none of the normal group. The RRM2 mRNA levels were significantly over expressed in epithelial ovarian cancer (1.722 ± 0.639) and borderline ovarian neoplasms (1.365 ± 0.615), compared to the normal group (0.678 ± 0.446) and benign group (0.828 ± 0.545). Patients with ovarian caner in clinical FIGO-stages III-IV presented higher RRM2 gene expression than those in clinical FIGO-stages I-II. Furthermore, the survival of patients with low RRM2 mRNA level was significantly better than patients with high levels (P < 0.05). By Cox proportional risk model analysis, the risk of mortality of patients with high level expression of RRM2 mRNA was 2.553 times greater than those with low expression.</p><p><b>CONCLUSION</b>RRM2 expression closely correlates with the development of ovarian tumor and may serve as a novel predictive marker for diagnosis and prognosis of the disease.</p>

Clinical predictive value of hemoglobin level in mortality of hospitalized COPD patients / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To further investigate an effect of hemoglobin level on organs and functions of chronic obstructive pulmonary diseases (COPD) patients with anemia, in order to provide clinical data on intervention for COPD patients with anemia.</p><p><b>METHODS</b>We retrospectively analyzed the predictive value of hemoglobin (Hb) level on the survival of inpatients, collected from 4960 COPD cases of three level of first-class hospitals.</p><p><b>RESULTS</b>(1) In 4960 COPD cases, there were 1009 cases with Hb < 110 g/L (males/females was 1009/4960, 20.34%); (2) The prevalence rate of pulmonary embolism (PE), congestive heart failure (CHF) and chronic renal failure (CRF) were significantly increased in the Hb level < 110 g/L and > or = 110 g/L group (P < 0.05); (3) Age, smoking, respiratory failure (RF), ischemic heart disease (IHD), pulmonary heart disease (PHD), CHF, atrial fibrillation (AF), PE, ARF, CRF, PaCO2, PaO2, and Hb levels were significantly different between survival and death group (P < 0.01); (4) Using stepwise regression analysis, age, smoking, Hb level, PaO2, RF, CRF, ACF, PHD, CHF and IHD had significantly association with death (P < 0.05); (5) Hb levels was significantly associated with death (95% CI 1.2975-2.8512 , P = 0.0014 ).</p><p><b>CONCLUSION</b>Low hemoglobin level (< 110 g/L) was significantly associated with death, and thus may be a valuable predictive marker for mortality of hospitalized COPD patients.</p>

Prevalence of hypertension and glucose/lipid metabolism disorders in 4960 inpatients with chronic obstructive pulmonary disease during 2000-2010 / 南方医科大学学报

<p><b>OBJECTIVE</b>To study the prevalence of hypertension and glucose/lipid metabolism induced by chronic obstructive pulmonary disease (COPD) in hospitalized patients and their correlations to age and gender.</p><p><b>METHODS</b>A retrospectively prevalence survey was conducted among the in-patients with COPD treated in General Hospital of PLA, Peking Union Medical College Hospital and Beijing Hospital between January 1st, 2000 and March 20th, 2010.</p><p><b>RESULTS</b>A total of 4960 COPD patients were selected, including 3570 male and 1390 female patients with a mean age of 72.2∓10.4 years. Among these patients, hypertension had the highest prevalence (40.3%), followed by diabetes/impaired glucose tolerance (18.8%) and then by hyperlipidemia/fat protein metabolism (6.9%). The prevalence of hyperlipidemia/lipoprotein metabolism in the COPD patients over 80 years old decreased significantly with age. The prevalence of hypertension increased with age. In patients over 50 years old, the prevalence of hypertension was significantly higher in female than in male patients (P<0.05). The prevalence of diabetes/impaired glucose tolerance increased with age in male patients but decreased in female patients aged :80 years (P<0.05). Female patients in 60-69.9 and 70-79.9 years groups had significantly higher incidence of diabetes/impaired glucose tolerance than male patients (P<0.05), but not at older ages. The incidence of hyperlipidemia/lipoprotein metabolism disorder increased with age in both male and female patients aged below 80 years (P<0.05), but in 60-69.9 and 70-79.9 year groups, female patients had a significantly higher incidence than male patients (P<0.05); at the ages :80 years, the incidences was lowered in both women and men without a gender-specific differences (P>0.05).</p><p><b>CONCLUSIONS</b>The data we obtained concerning the prevalence of hypertension and glucose/lipid metabolism disorder, age distribution, and gender characteristics of the COPD patients provide a clear target for secondary prevention of COPD.</p>

Retrospective study on the prevalence of cardiovascular comorbidities in 4960 inpatients with chronic obstructive pulmonary disease in Beijing / 中华流行病学杂志

Objective To retrospectively explore the prevalence of cardiovascular disease (CVD) in inpatients with chronic obstructive pulmonary disease(COPD)in Beijing.Methods The COPD patients who were discharged from the General Hospital of the Liberation Army,Peking Union medical college Hospital and Beijing Hospital between January 1st,2000 to March 20th,2010,were investigated.The prevalence of CVD were calculated.The tendency of the prevalence of CVD by age or discharge year and the difference of the prevalence of CVD between male and female were estimated by using chi-square analysis.Results There were 4960 COPD patients who were in accordance with the inclusion criteria with 3570 males and 1390 females.The mean age was 72.2±10.4 years.Of the COPD patients,48.8% were diagnosed as cardiovascular diseases.The age-adjusted over-all prevalence of CVD was 26.4%.Chronic pulmonary heart disease and other disease of pulmonary artery(15.8%)was the most frequent diseases,followed by heart failure(13.6%),ischemic heart disease (10.6%).In COPD patients,male was more likely to have angina,pulmonary heart disease and other disease of pulmonary artery and acute kidney failure (P＜0.05),while less likely to get arrhythmia (atrial fibrillation/atrial flutter),heart failure,pulmonary embolism,hypertension and diabetes mellitus (P＜0.05).The prevalence of arrhythmia increased with age,however,the ischemic heart disease and heart failure decreased.The proportion of CVD decreased in male patients while increased in females.Conclusion The overall prevalence of CVD comorbidities was 48.8% in 4960 patients with COPD who were older than 40 years in Beijing.There were differences among the groups of various age and sex in the distribution of CVD comorbidities frequencies year by year.